期刊
NANO LETTERS
卷 19, 期 4, 页码 2603-2613出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.9b00421
关键词
Au nanorods (AuNRs); aptamer; DNA nanodevice; dimerization; receptor tyrosine kinase
类别
资金
- National Natural Science Foundation of China [21725503, 21575038, 81730064, 81571985, 31571368]
- National Science and Technology Major Project [2017ZX10202201]
- Young Top-notch Talent for Ten Thousand Talent Program
- Keypoint Research and Invention Program of Hunan Province [2017DK2011]
- NIH [CA238042]
Optogenetics provides promising tools for the precise control of receptor-mediated cell behaviors in a spatiotemporal manner. Yet, most photoreceptors require extensive genetic manipulation and respond only to ultraviolet or visible light, which are suboptimal for in vivo applications because they do not penetrate thick tissues. Here we report a novel near-infrared light-activated DNA agonist (NIR-DA) nanodevice for nongenetic manipulation of cell signaling and phenotype in deep tissues. This nanodevice is prepared by conjugating a preinactivated DNA agonist onto the gold nanorods (AuNRs). Upon NIR light treatment, the DNA agonist is released through the localized surface plasmon resonance (LSPR)-based photothermal effect of AuNRs and becomes active. The active DNA agonist dimerizes the DNA-modified chimeric or native receptor tyrosine kinase (RTK) on cell surfaces and activates downstream signal transduction in live cells. Such NIR-DA activation of RTK signaling enables the control of cytoskeletal remodeling, cell polarization, and directional migration. Furthermore, we demonstrate that the NIR-DA system can be used in vivo to mediate RTK signaling and skeletal muscle satellite cell migration and myogenesis, which are critical cellular behaviors in the process of skeletal muscle regeneration. Thus, the NIR-DA system offers a powerful and versatile platform for exogenous modulation of deep tissues for purposes such as regenerative medicine.
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