期刊
NANO LETTERS
卷 19, 期 4, 页码 2641-2646出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.9b00508
关键词
Super-resolution microscopy; DNA nanotechnology; DNA-PAINT; barcoding multiplexing
类别
资金
- German Research Foundation through the Emmy Noether Program [DFG JU 2957/1-1]
- German Research Foundation [SFB1032]
- European Research Council through an ERC Starting Grant (MolMap) [680241]
- Allen Distinguished Investigator Program through the Paul G. Allen Frontiers Group
- Max Planck Society
- Max Planck Foundation
- NSF [CCF-1317291]
- NIH [1-U01-MH106011, R01EB018659]
- European Research Council (ERC) [680241] Funding Source: European Research Council (ERC)
Optical super-resolution techniques reach unprecedented spatial resolution down to a few nanometers. However, efficient multiplexing strategies for the simultaneous detection of hundreds of molecular species are still elusive. Here, we introduce an entirely new approach to multiplexed super-resolution microscopy by designing the blinking behavior of targets with engineered binding frequency and duration in DNA-PAINT. We assay this kinetic barcoding approach in silico and in vitro using DNA origami structures, show the applicability for multiplexed RNA and protein detection in cells, and finally experimentally demonstrate 124-plex super-resolution imaging within minutes.
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