期刊
NANO LETTERS
卷 19, 期 4, 页码 2267-2271出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.8b04673
关键词
Light-field metasurface; interleaved metasurface; single-particle tracking; Mie resonance; microlens array; point spread function engineering
类别
资金
- multiuniversity research grant (MURI) of the U.S. Airforce Office for Scientific Research [FA9550-14-1-0389]
- individual investigator grant of the U.S. Airforce Office for Scientific Research [FA9550-17-1-0331]
- National Defense Science and Engineering Graduate (NDSEG) Fellowship [32 CFR 168a]
Three-dimensional (3D) single-particle tracking (SPT) is a key tool for studying dynamic processes in the life sciences. However, conventional optical elements utilizing light fields impose an inherent trade-off between lateral and axial resolution, preventing SPT with high spatiotemporal resolution across an extended volume. We overcome the typical loss in spatial resolution that accompanies light-field-based approaches to obtain 3D information by placing a standard microscope coverslip patterned with a multifunctional, light-field metasurface on a specimen. This approach enables an otherwise unmodified microscope to gather 3D information at an enhanced spatial resolution. We demonstrate simultaneous tracking of multiple fluorescent particles within a large 0.5 x 0.5 x 0.3 mm(3) volume using a standard epi-fluorescent microscope with submicron lateral and micron-level axial resolution.
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