期刊
MOLECULES
卷 24, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/molecules24050933
关键词
two-component system; histidine kinase; luteolin; ATP; NMR; molecular docking; inhibition
资金
- National Key R&D Program of China [2017YFA0505400]
- National Science Foundation of China [21573280, 21603268, 21735007]
The two-component system (TCS) is a significant signal transduction system for bacteria to adapt to complicated and variable environments, and thus has recently been regarded as a novel target for developing antibacterial agents. The natural product luteolin (Lut) can inhibit the autophosphorylation activity of the typical histidine kinase (HK) HK853 from Thermotoga maritime, but the inhibition mechanism is not known. Herein, we report on the binding mechanism of a typical flavone with HK853 by using solution NMR spectroscopy, isothermal titration calorimetry (ITC), and molecular docking. We show that luteolin inhibits the activity of HK853 by occupying the binding pocket of adenosine diphosphate (ADP) through hydrogen bonds and pi-pi stacking interaction structurally. Our results reveal a detailed mechanism for the inhibition of flavones and observe the conformational and dynamics changes of HK. These results should provide a feasible approach for antibacterial agent design from the view of the histidine kinases.
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