期刊
ANALYTICAL CHEMISTRY
卷 88, 期 18, 页码 8931-8935出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b02834
关键词
-
资金
- National Science Foundation [CHE-1308114]
- National Institute of Health [1RO1GM106016]
- Walther Cancer Foundation
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1308114] Funding Source: National Science Foundation
Fatty acid (FA) profiling provides phenotypic information and is increasingly used in a broad,range of biological and, biomedical studies. Quantitation of unsaturated FAs with confident carbon-carbon, double bond (C=C) location assignment is both: sample and time consuming using-traditional gas chromatography mass spectrometry analysis. In this study, we-developed a rapid, sensitive, and quantitative method for profiling unsaturated FAs without using chromatographic-separations. This method was based on a combination of in-Solution photochemical tagging of a C=C;in FAs and a subsequent gas-phase detagging via tandem (neutral loss scan) mass spectrometry. It enabled quantitation of unsaturated FAs from, various biological samples (blood, plasma, and cell lines). More importantly, quantitative information on FA C=C location isomers, which was traditionally overlooked, could now be obtained and applied to studying FA changes between normal and cancerous human prostate cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据