期刊
MOLECULAR CELL
卷 74, 期 1, 页码 158-+出版社
CELL PRESS
DOI: 10.1016/j.molcel.2019.01.027
关键词
-
资金
- Wellcome Trust Investigator Award [107928/Z/15/Z]
- ERC Advanced Grant [339170]
- Marie Curie fellowship from EU FP7 [327985]
- Boehringer Ingelheim
- HFSP [CDA00066/2015]
- FWF (START project) [Y 1031-B28]
- Wellcome Trust [103768]
- ERC [340081]
- IMP
- European Research Council (ERC) [340081] Funding Source: European Research Council (ERC)
- Wellcome Trust [107928/Z/15/Z] Funding Source: Wellcome Trust
The pervasive nature of RNA polymerase II (Pol II) transcription requires efficient termination. A key player in this process is the cleavage and polyadenylation (CPA) factor PCF11, which directly binds to the Pol II C-terminal domain and dismantles elongating Pol II from DNA in vitro. We demonstrate that PCF11-mediated termination is essential for vertebrate development. A range of genomic analyses, including mNET-seq, 3' mRNA-seq, chromatin RNAseq, and ChIP-seq, reveals that PCF11 enhances transcription termination and stimulates early polyadenylation genome-wide. PCF11 binds preferentially between closely spaced genes, where it prevents transcriptional interference and consequent gene downregulation. Notably, PCF11 is sub-stoichiometric to the CPA complex. Low levels of PCF11 are maintained by an auto-regulatory mechanism involving premature termination of its own transcript and are important for normal development. Both in human cell culture and during zebrafish development, PCF11 selectively attenuates the expression of other transcriptional regulators by premature CPA and termination.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据