期刊
ANALYTICAL BIOCHEMISTRY
卷 503, 期 -, 页码 65-67出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2016.03.014
关键词
SOD; Hydroxylamine hydrochloride; Epinephrine; Nitroblue tetrazolium; Cytochrome c; WST
资金
- Natural Science Foundation of China [31071497, 31271859]
- National Basic Research Program of Science and Technology Ministry of China [2010CB530605]
To calculate superoxide dismutase (SOD) activity rapidly and accurately by indirect SOD assays, a formula based on the ratio of the catalytic speed of SOD to the reaction speed of the indicator with superoxide anion was deduced. The accuracy of this formula was compared with the conventional formula based on inhibition in five indirect SOD assays. The new formula was validated in nearly the entire SOD activity range, whereas the conventional formula was validated only during inhibition of 40-60%. This formula might also be used for the assays of other enzymes. (C) 2016 Elsevier Inc. All rights reserved.
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