4.7 Article

Protein Aggregation Capture on Microparticles Enables Multipurpose Proteomics Sample Preparation

期刊

MOLECULAR & CELLULAR PROTEOMICS
卷 18, 期 5, 页码 1027-1035

出版社

AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/mcp.TIR118.001270

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资金

  1. Novo Nordisk Foundation [NNF14CC0001]
  2. European Union's Horizon 2020 research and innovation program [686547]
  3. HOPE project grant from The Novo Nordisk Foundation [NNF17SA0027704]
  4. Marie Sklodowska-Curie European Training Network (ETN) TEMPERA - European Union's EU Framework Programme for Research and Innovation Horizon 2020 [722606]
  5. Lundbeck Foundation
  6. NEYE Foundation
  7. Danish Medical Research Council

向作者/读者索取更多资源

Universal proteomics sample preparation is challenging because of the high heterogeneity of biological samples. Here we describe a novel mechanism that exploits the inherent instability of denatured proteins for nonspecific immobilization on microparticles by protein aggregation capture. To demonstrate the general applicability of this mechanism, we analyzed phosphoproteomes, tissue proteomes, and interaction proteomes as well as dilute secretomes. The findings present a practical, sensitive and cost-effective proteomics sample preparation method. Molecular & Cellular Proteomics 18: 1027-1035, 2019. DOI: 10.1074/ mcp.TIR118.001270.

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