期刊
MITOCHONDRION
卷 46, 期 -, 页码 1-5出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.mito.2019.03.001
关键词
Fluorescence-based reporter; Mitochondria; Fusion; Proteases; OMA1
资金
- University of Arkansas for Medical Research Pilot Research Funds
- NIH [R01 GM106419]
- PhRMA Foundation Pre Doctoral Fellowship
- NIHT32 [GM106999]
Mitochondria continually undergo fission and fusion which allow mitochondria to rapidly change their shape, size, and function throughout the cell life cycle. OMA1, a zinc metalloproteinase enzyme, is a key regulator of the mitochondrial fusion machinery. The paucity of information regarding OMA1 regulation and function largely stems from the fact that there is no direct method to quantitatively measure its activity. Using a fluorescence-based reporter assay, we developed a sensitive method to measure OMA1 enzymatic activity in whole cell lysates.
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