期刊
MICROCHIMICA ACTA
卷 186, 期 3, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-019-3327-7
关键词
Metal nanocluster; Glutathione; Copper ion; Ratiometric nanoprobe; Oxidation reaction; Oxidized form of o-phenylenediamine; Fluorescence resonance energy transfer; Dynamic quenching
资金
- National Natural Science Foundation of China [21575043, 21275056, 21605052, 51478196]
- Platform Construction Project of Guangzhou Science Technology and Innovation Commission [15180001]
Copper nanoclusters (CuNCs) were synthesized starting from glutathione and copper(II) nitrate. They show blue fluorescence peaking at 432 nmwhen excited at 334 nm. In the presence of o-phenylenediamine (OPD), the blue fluorescence is decreased, but a yellow fluorescence appears with a peak at 557 nm. UV-visible absorptiometry, fluorometry and fluorescence lifetime measurements were used to show that OPD is oxidized by the small fraction of copper(II) ions present in the CuNCs to form the oxidized form of o-phenylenediamine (oxOPD) which displays weak yellow fluorescence, while the blue fluorescence decreases. The ratio of fluorescences at 557 and 432 nm increases linearly in the 0.15 to 110 mu g.L-1 OPD concentration range, and the detection limit is 93 ng.L-1. Compared to the method based on the use of dissolved Cu(II), the employment of CuNCs reduces the detection limit by a factor of 40. The method was applied to the determination of OPD in spiked environmental water and industrial wastewater samples. Recoveries ranged from 96.8 to 100.3%.
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