期刊
MICROCHIMICA ACTA
卷 186, 期 4, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-019-3331-y
关键词
Enzyme mimics; Nature enzyme; Ficin@MOF composites; Saucer-like structure; One-pot synthesis; H2O2; TMB
资金
- National Natural Science Foundation of China [21405124, 21175110]
- Fundamental Research Funds for the Central Universities [XDJK2013A022, XDJK2014C173, XDJK2017D052]
An enzyme-metal organic framework (MOF) composite with saucer-like structure was prepared via one-pot synthesis from ficin (a cysteine proteolytic enzyme with POx activity), zinc(II) ions and 2-methylimidazole. The composites exhibit a 2.5-fold higher catalytic activity and stronger affinity for substrates compared to free ficin. This was exploited to design a colorimetric assay for the determination of glucose. The addition of glucose oxidase causes the formation of H2O2 which is catalytically oxidized by ficin to form a blue coloration that can be measured at 652nm. The assay has a 0.12M detection limit and excellent selectivity. It was successfully applied to the determination of glucose in diluted serum samples.
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