期刊
MICROCHIMICA ACTA
卷 186, 期 4, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-019-3346-4
关键词
Global phosphoproteome; Immobilization metal ion affinity chromatography; Metal oxide affinity chromatography; Cataract; Functionalized magnetic nanomaterials
资金
- National Key RAMP
- D Program of China [2018YFA0507501]
- National Natural Science Foundation of China [21425518]
The authors are presenting a novel strategy for global phosphoproteome recognition in practical samples. It integrates metal oxide affinity chromatography (MOAC) and immobilization metal ion affinity chromatography (IMAC). This resulted in a kind of titanium dioxide/ion-based multifunctional probe (dubbed T2M). The T2M combines the features of MOAC and IMAC including their recognition preferences towards mono- and multi-phosphorylated peptides. Hence, they exhibit an outstanding recognition capability towards global phosphoproteome, high sensitivity (the limit of detection of which is merely 10 fmol) and excellent specificity in MALDI-TOF MS detection. Their performance is further demonstrated by the identification of the phosphoproteome in non-fat milk and human saliva. By combining T2M with nano LC-MS/MS, remarkable results are obtained in the tryptic digestion of healthy eye lens and cataract lens phosphoproteomes. A total of 658 and 162 phosphopeptides, respectively, were identified. This indicates that phosphorylation and the appearance of cataract can be related to each other.
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