期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 408, 期 21, 页码 5737-5743出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-016-9675-9
关键词
Gonyautoxins 1,4; Molecularly imprinted polymers; Solid-phase extraction; Alexandrium minutum; Alexandrium tamarense
资金
- National Programme on Global Change and Air-Sea Interaction [GASI-03-01-02-01]
- Public Science and Technology Research Funds Projects of Ocean [201505034]
A kind of new molecularly imprinted polymer (MIP) was synthesized by bulk polymerization using guanosine as dummy template molecule, alpha-methacrylic acid as functional monomer and ethylene glycol dimethyl acrylic ester as crosslinker. Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) showed that the MIP had homogenous and uniform-sized cavities. It was confirmed that the MIP had higher binding affinity and selectivity towards gonyautoxins 1,4 (GTX 1,4) than the non-imprinted polymer (NIP) according to the static equilibrium adsorption. An off-line molecularly imprinted solid-phase extraction (MISPE) method followed by high-performance liquid chromatography with fluorescence detection (HPLC-FLD) was established for the analysis of GTX 1,4. 0.1 mol/L acetic acid and 95:5 (v:v) methanol/water were optimized as the washing and elution solutions, respectively. The recoveries of spiked cultured seawater samples were satisfactory, as high as 88 %. Using this method, the concentrations of GTX 1,4 from cultured seawater samples of Alexandrium minutum and Alexandrium tamarense were detected to be 1.10 mu g/L and 0.99 mu g/L, respectively.
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