期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 408, 期 20, 页码 5633-5640出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-016-9667-9
关键词
Localized surface plasmon resonance; Glycopolymer brush; Microfluidics; Bacterial toxin; Glycosyltransferase; Biosensors
资金
- Deutsche Forschungsgemeinschaft (DFG) [SFB 985]
A localized surface plasmon resonance biosensor in a flow-through configuration was applied for investigating kinetics of lectin binding to surface-grafted glycopolymer brushes. Polycarbonate filter membranes with pore sizes of 400 nm were coated with a 114-nm thick gold layer and used as substrate for surface-initiated atom-transfer radical polymerization of a glycomonomer. These grafted from glycopolymer brushes were further modified with two subsequent enzymatic reactions on the surface to yield an immobilized trisaccharide presenting brush. Specific binding of lectins including Clostridium difficile toxin A receptor domain to the glycopolymer brush surface could be investigated in a microfluidic setup with flow-through of the analytes and transmission surface plasmon resonance spectroscopy.
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