期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 408, 期 15, 页码 4159-4168出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-016-9507-y
关键词
endo-1,4-beta-Glucanase; Cellulase; Assay; Colorimetric; CELLG5; Automation
endo-1,4-beta-Glucanase (endo-cellulase, EC 3.2.1.4) is one of the most widely used enzymes in industry. Despite its importance, improved methods for the rapid, selective, quantitative assay of this enzyme have been slow to emerge. In 2014, a novel enzyme-coupled assay that addressed many of the limitations of the existing assay methodology was reported. This involved the use of a bifunctional substrate chemically derived from cellotriose. Reported herein is a much improved version of this assay employing a novel substrate, namely 4,6-O-(3-ketobutylidene)-4-nitrophenyl-beta-d-cellopentaoside.
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