期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 408, 期 24, 页码 6703-6709出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-016-9787-2
关键词
Nitrofuran metabolites; Magnetic separation; Lateral flow biosensor; Fast pre-treatment
资金
- Science & Technology Project in Guangzhou Zhujiang Science & Technology New Star [2014j2200080]
- Funds for Public Welfare Research in Guangdong Province [2014B020212011]
- Chinese Academy of Sciences [2012B091100267]
- Guangdong Province [2012B091100267]
The use of potential mutagenic nitrofuran antibiotic in food animal production has been banned world-wide. Common methods for nitrofuran detection involve complex extraction procedures. In the present study, magnetic beads functionalized with antibody against nitrofuran derivative were used as both the extraction and color developing media in lateral flow biosensor. Derivatization reagent carboxybenzaldehyde is firstly modified with ractopamine. After reaction with nitrofuran metabolites, the resultant molecule has two functional groups: the metabolite moiety and the ractopamine moiety. Metabolite moiety is captured by the antibody that is coated on magnetic beads. This duplex is then loaded onto biosensor and ractopamine moiety is further captured by the antibody immobilized on the test zone of nitrocellulose membrane. Without tedious organic reagent-based extraction procedure, this biosensor was capable of visually detecting four metabolites simultaneously with a detection limit of 0.1 mu g/L. No cross-reactivity was observed in the presence of 50 mu g/L interferential components.
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