期刊
LETTERS IN APPLIED MICROBIOLOGY
卷 68, 期 5, 页码 446-454出版社
WILEY
DOI: 10.1111/lam.13123
关键词
1-phenyl-1; 2-ethanediol; 2-hydroxyacetophenone; asymmetric oxidation; Kurthia gibsonii; whole-cell biocatalysis
资金
- National Natural Science Foundation of China [21676104, 21878105]
- National Key Research and Development Program of China [2018YFC1603403]
- Science and Technology Program of Guangzhou
Chiral 1-phenyl-1,2-ethanediol (PED) performs vital effect for the preparation of pharmaceuticals, agrochemicals and cosmetics. In the study, a newly isolated strain Kurthia gibsoniiSC0312 with the ability to selectively oxidize racemic PED to achieve (S)-PED was evaluated in the aqueous reaction system. The strain showed excellent catalytic performances within the range of pH 55-85, temperature 25-45 degrees C and the amount of cell 15mgml(-1) to 30mgml(-1). Besides, 2-hydroxyacetophenone (HAP) as the oxidation product displayed a stronger inhibition to the catalytic activity of cell, only remaining <63% of catalytic activity after incubation at 40mmoll(-1) HAP for 6h. For various metal ions, Cu2+ can obviously improve 17 times of the catalytic activity of cell at the concentration of 02mmoll(-1). Acetone can stimulate the catalytic capacity of cell to improve the optical purity of (S)-PED at the PED concentration of 80mmoll(-1), up to appropriately 94% from 854%; compared to the resting cell, growing cell exerted no positive effect in the yield and optical purity. Finally, a highly effective kinetic resolution system of racemic PED by the new strain was obtained, with the (S)-PED yield of 41% and optical purity of 94%.
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