期刊
ANALYTICA CHIMICA ACTA
卷 924, 期 -, 页码 21-28出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2016.04.011
关键词
Mediated cell-based electrochemical biosensor; Mixed microbial consortium; Toxic unit; Joint toxicity
资金
- International Science & Technology Cooperation Program of China [2013DFG50150]
- Natural Foundation of Sciences of the People's Republic of China [21175144]
- Key Project of Beijing Natural Science Foundation [2120002]
Since most risk assessment for toxicants is based on individual single-species test, the deduction of such results to ecosystem evaluation is afflicted with uncertainties. Herein, we successfully developed a pbenzoquinone mediated whole-cell electrochemical biosensor for multi-pollutants toxicological analysis by co-immobilizing mixed strains of microorganism, including Escherichia coli (gram-negative bacteria), Bacillus subtilis (gram-positive bacteria) and Saccharomyces cerevisiae (fungus). The individual and combined toxicities of heavy metal ions (Cu2+, Cd2+), phenol (3,5-dichlorophenol) and pesticides ( Ametryn, Acephate) were examined. The experimental results showed that the order of toxicity for individual toxicant was ranked as Cu2+ > 3,5-dichlorophenol (DCP) > Ametryn > Cd2+ > Acephate. Then the toxic unit (TU) model was applied to determine the nature of toxicological interaction of the toxicants which can be classified as concentration additive (IC50(mix) = 1TU), synergistic (IC50(mix) < 1TU) and antagonistic (IC50(mix) > 1TU) responses. The binary combination of Cu2+ + Cd2+, Cu2+ + DCP, Cu2+ + Acephate, DCP + Acephate, Acephate + Ametryn were analyzed and the three kind of joint toxicity effects (i.e. additive, synergistic and antagonistic) mentioned above were observed according to the dose-response relationship. The results indicate that the whole-cell electrochemical biosensor based on mixed microbial consortium is more reasonable to reflect the joint biotoxicity of multi-pollutants existing in real wastewater, and combined effects of toxicants is extremely necessary to be taken into account in ecological risk assessment. Thus, present study has provided a promising approach to the quality assessment of wastewater and a reliable way for early risk warning of acute biotoxicity. (C) 2016 Elsevier B.V. All rights reserved.
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