期刊
JOURNAL OF VIROLOGICAL METHODS
卷 264, 期 -, 页码 61-64出版社
ELSEVIER
DOI: 10.1016/j.jviromet.2018.12.003
关键词
DNA extraction; Frass; Faeces; Crickets; Acheta domesticus; Densovirus; AdDV
资金
- Swedish Agricultural Research Council (FORMAS) [2016-00361]
- Vinnova [2016-00361] Funding Source: Vinnova
- Formas [2016-00361] Funding Source: Formas
The European house cricket (Acheta domesticus) is a species of interest for the emerging inseot-as-food industry. Acheta domesticus densovirus (AdDV) is a member of the Parvoviridae virus family which infects A. domesticus, causing widespread mortality and even extinction of local cricket populations. Despite the well-known detrimental effects of AdDV in commercial rearing of A. domesticus there are no optimized protocols to accurately and non-destructively detect and quantify the virus. This study establishes a new protocol for the detection of AdDV in faecal material from A.domesticus. The protocol includes methodological improvements, such as upgrading from conventional PCR to quantitative real-time PCR and is much more sensitive than previously published protocols. Moreover, this study shows that cricket faeces are a suitable, non-destructive sample substrate to infer reliably if a cricket population is infected with AdDV or not. Early detection of lethal or economic threats, such as disease-causing viruses, is an essential part of commercial cricket management as well as for monitoring the risk of spread to wild cricket populations or to (human) consumers.
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