期刊
ANALYTICA CHIMICA ACTA
卷 938, 期 -, 页码 1-6出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2016.07.035
关键词
Biomarkers; Metal-doped magnetic nanoparticles; Immunoassay; ICP-MS; Multiplex detection
资金
- Nano-Convergence Foundation (Development of immuno-biomaterial analyzer using engineered nanomaterials) - Ministry of Science, ICT and Future Planning (MSIP, Korea)
- Ministry of Trade, Industry and Energy (MOTIE, Korea)
- Korean National Research Foundation [NRF-2015R1D1A1A01057721]
- National Research Council of Science & Technology (NST), Republic of Korea [KK-1604] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2015R1D1A1A01057721] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Metal-doped inorganic nanoparticles were synthesized for the multiplex detection of biomarkers by a sandwich-type inductively coupled plasma mass spectrometry (ICP-MS) immunoassay. The synthesized Cs-doped multicore magnetic nanoparticles (MMNPs) were used not only for magnetic extraction of targets but also for ratiometric measurement in ICP-MS. In addition, three different metal/dye-doped silica nanoparticles (SNPs) were synthesized as probes for multiplex detection: Y/RhBITC (rhodamine B isothiocyanate)-doped SNPs for CRP (cardiovascular disease), Cd/RhBITC-doped SNPs for AFP (tumor), and Au/5(6)-XRITC (X-rhodamine-5-(and-6)-isothiocyanate)-doped SNPs for NSE (heart disease). For quantification, the doped metals of SNPs were measured. by ICP-MS and then the signal ratio to Cs of MMNPs was plotted with respect to the concentration of targets by a ratiometry. Limits of detection (LOD) of 0.35 ng/mL to 77 ng mL(-1) and recoveries of 83%-125% were obtained for serum samples spiked with the biomarkers. Since no sample treatment was necessary prior to the extraction, the proposed method provided short analysis time and convenience for the multiplex determination of biomarkers, which will be valuable for clinical application. (C) 2016 Elsevier B.V. All rights reserved.
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