期刊
JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
卷 82, 期 3, 页码 179-185出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/JCMA.0000000000000027
关键词
Monocytes; Neuraminidase; Sialyltransferases
资金
- Chang Gung Medical Foundation, Taiwan [CMRPG3C1641, CMRPG390162]
Background: We decided to study the association of monocyte alpha-2,3-sialyltransferase 1 (ST3Gal-1), neuraminidase-3 (Neu3), alpha-2,6-sialyltransferase 1 (ST6Gal-1), and neuraminidase-1 (Neu1) levels with disease activity score 28 (DAS28) in human rheumatoid arthritis (RA), considering that mouse monocytes' sialic acid (SIA) levels relate to their phagocytosis and IgG binding ability. Methods: ST3Gal-1, Neu3, ST6Gal-1, Neu1, alpha-2,3-SIA, and alpha-2,6-SIA levels on RA peripheral blood monocytes, T cells, and polymorphonuclear cells were determined by using fluorochrome-conjugated anti-cell-specific marker antibodies and fluorochrome-conjugated anti-enzyme antibodies. Simple correlation and linear regression were used to correlate enzyme levels with DAS28. Results: RA monocyte ST3Gal-1 and Neu3 levels correlated with DAS28 in patients having DAS28 >5.1 (r = 0.469, p = 0.002; r = 0.410, p = 0.006, respectively). When multivariable analysis was performed for erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and SIA-related enzyme levels in different cell types as independent variables with DAS28 as a dependent variable, monocyte ST3Gal-1 levels correlated with DAS28 (p = 0.009) but not ESR and CRP in patients having DAS28 >5.1 (both p >= 0.292). RA monocyte ST3Gal-1 levels correlated with DAS28 (p = 0.010) and with ESR (p < 0.001) at month 0 when applied to all RA patients including both remission and nonremission groups in multivariable analysis. The latter findings persisted longitudinally at month 3. Conclusion: Monocyte ST3Gal-1 and Neu3 levels correlated longitudinally with DAS28 by two different methods suggest that monocyte ST3Gal-1 and Neu3 levels may be used as biomarkers to monitor RA disease activity.
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