EM boolean AND IM: software for relating ion mobility mass spectrometry and electron microscopy data

We present EM boolean AND IM, software that allows the calculation of collision cross-sections from electron density maps obtained for example by means of transmission electron microscopy. This allows the assessment of structures other than those described by atomic coordinates with ion mobility mass spectrometry data, and provides a new means for contouring and validating electron density maps. EM boolean AND IM thereby facilitates the use of data obtained in the gas phase within structural biology studies employing diverse experimental methodologies.