期刊
JOURNAL OF SURFACTANTS AND DETERGENTS
卷 22, 期 3, 页码 477-490出版社
WILEY
DOI: 10.1002/jsde.12266
关键词
Candida antarctica lipase B; Silica microspheres; Immobilization; Cross-linked enzyme aggregates; Rhamnolipids
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Sri Ramaswamy Memorial (SRM) Institute of Science and Technology for the Selective Excellence Project
In the present study, Candida antarctica lipase B was immobilized on amine-functionalized silica microspheres as cross-linked enzyme aggregates (CLEA) and utilized for the biomanufacturing of rhamnolipids (RL). Lipase CLEA synthesized under optimized conditions of 2.0:1.0 by volume of silica microsphere/enzyme concentration, a 1.0:2.5 (v/v) ratio of enzyme/2-propanol, 7 mM glutaraldehyde concentration, when incubated at pH 9.0 and 40 degrees C, for a cross-linking time of 30 min were observed to exhibit superior biocatalytic properties and a maximum enzyme load of 770 U g(-1). Lipase CLEA exhibited enhanced pH stability in acidic and alkaline media and increased temperature resistance as compared to free lipase. Both free and CLEA lipases were used to synthesize RL in different solvent systems. After 12 h, from initiation of the esterification, the degree of esterification (molar conversion yield) reached 46% and 71% in the batch mode. H-1 and C-13 nuclear magnetic resonance (NMR) and high-performance liquid chromatographic (HPLC) analysis confirm RL production by CLEA lipase. The CLEA showed greater confrontation to enzyme-mediated bioprocess approach as compared to its soluble counterpart and exhibited excellent RL production and catalytic activity even after its tenth successive reuse.
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