期刊
JOURNAL OF PROTEOME RESEARCH
卷 18, 期 5, 页码 2021-2031出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.8b00911
关键词
proteomics; high altitude; hypobaric hypoxia; complement and coagulation cascades; antioxidant; glycolysis
资金
- PUMC Youth Fund
- Fundamental Research Funds for the Central Universities [2017310036]
- CAMS Innovation Fund for Medical Sciences [2016-12M-1-018, 2017-I2M-3-021]
- Department of Science and Technology of Sichuan Province [2019YFH0018, 2019YFS0308]
- Sichuan Medical Association [S17082]
For individuals migrating to or residing permanently in high-altitude regions, environmental hypobaric hypoxia is a primary challenge that induces several physiological or pathological responses. It is well documented that human beings adapt to hypobaric hypoxia via some protective mechanisms, such as erythropoiesis and overproduction of hemoglobin; however, little is known on the alterations of plasma proteome profiles in accommodation to high altitude hypobaric hypoxia. In the present study, we investigated differential plasma proteomes of high altitude natives and lowland normal controls by a TMT-based proteomic approach. A total of 818 proteins were identified, of which 137 were differentially altered. Bioinformatics (including GO, KEGG, protein protein interactions, etc.) analysis showed that the differentially altered proteins were basically involved in complement and coagulation cascades, antioxidative stress, and glycolysis. Validation results demonstrated that CCL18, C9, PF4, MPO, and S100A9 were notably up-regulated, and HRG and F11 were down-regulated in high altitude natives, which were consistent with TMT-based proteomic results. Our findings highlight the contributions of complement and coagulation cascades, antioxidative stress, and glycolysis in acclimatization to hypobaric hypoxia and provide a foundation for developing potential diagnostic or/and therapeutic biomarkers for high altitude hypobaric hypoxia-induced diseases.
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