期刊
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
卷 10, 期 5, 页码 1146-1151出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jpclett.9b00227
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类别
资金
- National Institutes of Health [CA35635, GM077422]
Circular dichroism and stopped-flow UV spectroscopies were used to investigate the thermodynamic stability and the folding pathway of d[TGAG(3)TG(3)TAG(3)TG(3)TA(2)] at 25 degrees C in solutions containing 25 mM KCl. Under these conditions the oligonucleotide adopts a thermally stable, all-parallel G-quadruplex topography containing three stacked quartets. K+-induced folding shows three resolved relaxation times, each with distinctive spectral changes. Folding is complete within 200 s. These data indicate a folding pathway that involves at least two populated intermediates, one of which seems to be an antiparallel structure that rearranges to the final all-parallel conformation. Molecular dynamics reveals a stereochemically plausible folding pathway that does not involve complete unfolding of the intermediate. The rate of unfolding was determined using complementary DNA to trap transiently unfolded states to form a stable duplex. As assessed by 1D-H-1 NMR and fluorescence spectroscopy, unfolding is extremely slow with only one observable rate-limiting relaxation time.
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