期刊
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
卷 29, 期 2, 页码 179-190出版社
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1807.06066
关键词
Bacillus subtilis; spore surface display technology; biocatalysis; gene engineering; anchor protein; linker peptide
资金
- National Key R & D Program of China [2017YFC1600806]
- National Natural Science Foundation of China [31571806]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province of China [SJCX18_0750]
- High-level talents project of Six Talent Peaks in Jiangsu Province [SWYY-018]
- China Postdoctoral Science Foundation [2017M621657]
Bacillus subtilis spore surface display (BSSD) technology is considered to be one of the most promising approaches for expressing heterologous proteins with high activity and stability. Currently, this technology is used for various purposes, such as the production of enzymes, oral vaccines, drugs and multimeric proteins, and the control of environmental pollution. This paper presents an overview of the latest developments in BSSD technology and its application in protein engineering. Finally, the major limitations of this technology and future directions for its research are discussed.
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