Intestinibaculum porci gen. nov., sp. nov., a new member of the family Erysipelotrichaceae isolated from the small intestine of a swine

A strictly anaerobic, Gram-stain-positive, catalase-negative, non-motile, rod-shaped bacterium, designated SG0102(T), was isolated from the small intestine of a swine. Optimal growth occurred at 37 degrees C and pH 7.0. Furthermore, growth was observed in the presence of up to 3% (w/v) NaCl but not at salinity levels higher than 4%. The comparative analysis of 16S rRNA gene sequences showed that strain SG0102(T) was most closely related to Kandleria vitulina DSM 20405(T) (93.3%), followed by Catenibacterium mitsuokai KCTC 5053(T) (91.1%), Sharpea azabuensis KCTC 15217(T) (91.0%), and Eggerthia catenaformis DSM 5348(T) (89.6%). The average nucleotide identity values between strain SG0102(T) and related species, K. vitulina DSM 20405(T), C. mitsuokai KCTC 5053(T), S. azabuensis KCTC 15217(T), and E. catenaformis DSM 5348(T), were 71.0, 69.3, 70.0, and 69.2%, respectively. The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain SG0102(T) belonged to the family Erysipelotrichaceae in the class Erysipelotrichia. The DNA G + C content of the strain SG0102(T) was 39.5 mol%. The major cellular fatty acids (> 10%) of strain SG0102(T) were C-16:0, C-16:0 dimethyl acetal, and C-18:29/12c. The cell wall peptidoglycan of strain SG0102(T) contained the meso-diaminopimelic acid. The strain SG0102(T) produced lactic acid as a major end product of fermentation. These distinct phenotypic and phylogenetic properties suggest that strain SG0102(T) represents a novel species in a novel genus of the family Erysipelotrichaceae, for which the name Intestinibaculum porci gen. nov. sp. nov. is proposed. The type strain is SG0102(T) (= KCTC 15725(T) = NBRC 113396(T)).