期刊
ANAEROBE
卷 39, 期 -, 页码 114-116出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.anaerobe.2016.03.013
关键词
mcrA; Community fingerprinting; BstNl; Mwol; Methanogenic archaea; Biogas
类别
资金
- Initiative and Networking Fund of Helmholtz Association
We present a simple protocol for the cost- and time-efficient profiling of methanogens based on T-RFLP fingerprinting of mcrA amplicons. Sequence data were compiled from mesophilic lab-scale and full-scale biogas reactors operated under various conditions and fed with various substrates. The database facilitates the rapid identification of methanogens, thus reducing the need of cloning and sequencing. (C) 2016 Elsevier Ltd. All rights reserved.
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