期刊
JOURNAL OF LEUKOCYTE BIOLOGY
卷 105, 期 6, 页码 1155-1165出版社
WILEY
DOI: 10.1002/JLB.MA0818-308R
关键词
IL-23; neutrophils; Th17 cells; TLR8; TNF-alpha
资金
- Associazione Italiana per la Ricerca sul Cancro (AIRC) [IG-20339]
- Ministero dell'Istruzione, dell'Universita e della Ricerca [MIUR-PRIN 2015YYKPNN]
- Aintree Arthritis Trust (Liverpool)
Human neutrophils contribute to the regulation of inflammation via the generation of a range of cytokines that affect all elements of the immune system. Here, we investigated their ability to express some of the members of the IL-12 family after incubation with TLR8 agonists. Highly pure human neutrophils were thus incubated for up to 48 h with or without R848, or other TLR8 agonists, to then measure the expression levels of transcripts and proteins for IL-12 family member subunits by RNA-seq, reverse transcription quantitative PCR, and ELISA. We show a TLR8-mediated inducible expression of IL-12B and IL-23A, but not IL-12A, mRNA, which occurs via chromatin remodeling (as assessed by ChIP-seq), and subsequent production of IL-23 and IL-12B, but no IL-12, proteins. Induction of IL-23 requires endogenous TNF-alpha, as both mRNA and protein levels were blocked in TLR8-activated neutrophils via a TNF-alpha-neutralizing Ab. We also show that supernatants from TLR8-activated neutrophils, but not autologous monocytes, induce the differentiation of Th17 cells from naive T cells in an IL-23-dependent fashion. This study unequivocally demonstrates that highly pure human neutrophils express and produce IL-23, further supporting the key roles played by these cells in the important IL-17/IL-23 network and Th17 responses.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据