期刊
JOURNAL OF INVESTIGATIVE DERMATOLOGY
卷 139, 期 7, 页码 1497-+出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jid.2019.01.011
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类别
资金
- EB Research Partnership
- EB Medical Research Foundation
- Office of the Assistant Secretary of Defense for Health Affairs
- Defense Health Agency J9, Research and Development Directorate, through the Congressionally Directed Medical Research Program [W81XWH-18-1-0382]
Mutations in the gene encoding collagen VII cause the devastating blistering disease recessive dystrophic epidermolysis bullosa (RDEB). RDEB is characterized by severe skin fragility and nonhealing wounds aggravated by scarring and fibrosis. We previously showed that TSP1 is increased in RDEB fibroblasts. Because transforming growth factor-beta (TGF-beta) signaling is also increased in RDEB, and TSP1 is known to activate TGF-beta, we investigated the role of TSP1 in TGF-beta signaling in RDEB patient cells. Knockdown of TSP1 reduced phosphorylation of smad3 (a downstream target of TGF-beta signaling) in RDEB primary fibroblasts, whereas overexpression of collagen VII reduced phosphorylation of smad3. Furthermore, inhibition of TSP1 binding to the LAP/TGF-beta complex decreased fibrosis in engineered extracellular matrix formed by RDEB fibroblasts, as evaluated by picrosirius red staining and analyses of birefringent collagen fibrillar deposits. We show that collagen VII binds TSP1, which could potentially limit TSP1-LAP association and subsequent TGF-beta activation. Our study suggests a previously unreported mechanism for increased TGF-beta signaling in the absence of collagen VII in RDEB patient skin. Moreover, these data identify TSP1 as a possible target for reducing fibrosis in the tumor-promoting dermal microenvironment of RDEB patients.
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