期刊
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
卷 61, 期 12, 页码 1194-1200出版社
WILEY
DOI: 10.1111/jipb.12800
关键词
-
资金
- Basic Center Project of National Natural Science Foundation of China [31788103]
- Chinese Ministry of Agriculture [2016ZX08009003-002]
Calcineurin B-like interacting protein kinases (CIPKs) play important roles via environmental stress. However, less is knownhowto sense the stress in molecular structure conformation level. Here, an OsCIPK7 mutant via TILLING procedure with a point mutation in the kinase domain showed increased chilling tolerance, which could be potentially used in the molecular breeding. We found that this point mutation of OsCIPK7 led to a conformational change in the activation loop of the kinase domain, subsequently with an increase of protein kinase activity, thus conferred an increased tolerance to chilling stress.
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