期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 373, 期 -, 页码 716-724出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2019.03.109
关键词
Carbon sources; Dechlorination; High-throughput sequencing; Bacterial community composition; Bacterial co-occurrence network
资金
- Guangdong Natural Science Funds for Distinguished Young Scholars [2016A030306019]
- National Natural Science Foundation of China [41877038]
- GDAS' Project of Science and Technology Development [2017GDASCX-0407]
- Natural Science Foundation of Guangdong Province [2018A030313385]
- Distinguished Youth Scholar Program of Jiangsu Province [BK20180049]
- Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program [2017BT01Z176]
- Youth Innovation Promotion Association of CAS [2017301]
In this study, experiments were performed using network analysis to investigate the effects of different carbon sources, including blank, citrate, glucose and lactate, on indigenous bacterial communities and on the pentachlorophenol (PCP) dechlorination in two soils. Kinetics results demonstrate that PCP dechlorination is significantly enhanced by adding citrate/lactate, but to a lesser extent by adding glucose. High-throughput sequencing results revealed that Firmicutes and Proteobacteria were the dominant groups in these four different treatments during the PCP dechlorination, whereas random forest analysis indicated that the orders Clostridiales, Haloplasmatales, Bacillales, Pseudomonodales and Gaiellales were the critical bacterial orders in modules that were significantly correlated with PCP dechlorination. Among them, the relative abundance of Clostridiales dramatically increased in both citrate and lactate treatment, further accelerating the PCP dechlorination. Addition of citrate/lactate as the carbon source increased the bacterial co-occurrence network density, average clustering coefficient and modularity. Moreover, more modules significantly correlated with PCP dechlorination in the citrate/lactate networks compared with the glucose/blank networks. Random forest modeling suggested that Clostridiales played a critical role in these functional modules. Taken together, our results provide insight into the biological mechanism of the impact of exogenous carbon sources on PCP dechlorination pathways by modifying soil bacterial networks.
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