期刊
JOURNAL OF FLUORESCENCE
卷 29, 期 2, 页码 425-434出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10895-019-02351-x
关键词
Fluorescent probe; Rhodamine; Fe3+; Cell imaging; DFT calculations
资金
- National Natural Science Foundation of China [21042002]
A new turn-on fluorescent probe Py based on rhodamine and piperonaldehyde was designed and synthesized for detecting Fe3+ in cells. The free probe Py was non-fluorescent. While only upon addition of Fe3+, the significant increase of the fluorescence and color were observed which could be visible directly by naked-eye. The probe Py shows high selectivity and sensitivity for Fe3+ over other common metal ions in EtOH-H2O (3/2, v/v) mixed solution. The association constant and the detection limit were calculated to be 4.81x10(4)M(-1) and 1.18x10(-8)mol/L respectively. The introduction of piperonaldehyde unit could increase probe rigidity which could enhance its optical properties. Meanwhile, the binding mode between Py and Fe3+ was found to be a 1:1 complex formation. The density functional theory (DFT) calculations were performed which would further confirm the recognition mechanism between probe Py and Fe3+. In addition, the probe has been proved to be reversible for detecting Fe3+. Moreover, the probe Py was used to detect Fe3+ in cells successfully.
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