期刊
JOURNAL OF EXPERIMENTAL MEDICINE
卷 216, 期 4, 页码 867-883出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20182192
关键词
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资金
- National Institutes of Health [AR067135, AI134877, GM113079, AR070918]
- Cancer Prevention and Research Institute of Texas [RP180288]
- Burroughs Wellcome Fund
STING gain-of-function mutations cause lung disease and T cell cytopenia through unknown mechanisms. Here, we found that these mutants induce chronic activation of ER stress and unfolded protein response (UPR), leading to T cell death by apoptosis in the Sting(N153S/+) mouse and in human T cells. Mechanistically, STING-N154S disrupts calcium homeostasis in T cells, thus intrinsically primes T cells to become hyperresponsive to T cell receptor signaling-induced ER stress and the UPR, leading to cell death. This intrinsic priming effect is mediated through a novel region of STING that we name the UPR motif, which is distinct from known domains required for type I IFN signaling. Pharmacological inhibition of ER stress prevented Sting(N153S/+) T cell death in vivo. By crossing Sting(N153S/+) to the OT-1 mouse, we fully restored CD8(+) T cells and drastically ameliorated STING-associated lung disease. Together, our data uncover a critical IFN-independent function of STING that regulates calcium homeostasis, ER stress, and T cell survival.
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