期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 70, 期 12, 页码 3089-3099出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erz131
关键词
Cuticular waxes; drought tolerance; DUF538; glossy mutant; glossy6 (gl6); maize (Zea mays)
资金
- National Key Research and Development Program of China [2016YFD0101002]
- 948 project of the Ministry of Agriculture of China [2015Z11]
- Agricultural Science and Technology Innovation Program of CAAS
- National Natural Science Foundation of China [31701437]
- Iowa State University
- Kansas Agricultural Experiment Station of Kansas State University
Cuticular waxes, long-chain hydrocarbon compounds, form the outermost layer of plant surfaces in most terrestrial plants. The presence of cuticular waxes protects plants from water loss and other environmental stresses. Cloning and characterization of genes involved in the regulation, biosynthesis, and extracellular transport of cuticular waxes onto the surface of epidermal cells have revealed the molecular basis of cuticular wax accumulation. However, intracellular trafficking of synthesized waxes to the plasma membrane for cellular secretion is poorly understood. Here, we characterized a maize glossy (gl6) mutant that exhibited decreased epicuticular wax load, increased cuticle permeability, and reduced seedling drought tolerance relative to wild-type. We combined an RNA-sequencing-based mapping approach (BSR-Seq) and chromosome walking to identify the gl6 candidate gene, which was confirmed via the analysis of multiple independent mutant alleles. The gl6 gene represents a novel maize glossy gene containing a conserved, but uncharacterized, DUF538 domain. This study suggests that the GL6 protein may be involved in the intracellular trafficking of cuticular waxes, opening the door to elucidating the poorly understood process by which cuticular wax is transported from its site of biosynthesis to the plasma membrane.
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