4.6 Article

Characterization of γδT Cells in Intestinal Mucosa From Patients With Early-Onset or Long-Standing Inflammatory Bowel Disease and Their Correlation With Clinical Status

期刊

JOURNAL OF CROHNS & COLITIS
卷 13, 期 7, 页码 873-883

出版社

OXFORD UNIV PRESS
DOI: 10.1093/ecco-jcc/jjz015

关键词

gamma delta T cells; inflammatory bowel disease; IL-17; IFN-gamma; clinical correlation

资金

  1. IG-IBD on voluntary donation of MSD Italia

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Background and Aims: Inflammatory bowel disease [IBD] is a complex chronic inflammatory disease of the human gut with no clear aetiology. Traditionally, dysregulated adaptive immune responses play an important role even though accumulating evidence suggests a role also for innate immunity. Because of the well-known plasticity of gamma delta T cells, we investigated their percentage occurrence, phenotypic features and effector functions in the intestinal mucosa of early-onset and long-standing IBD patients, as compared to healthy subjects. Methods: Fresh biopsies from 30 Crohn's disease and ulcerative colitis patients were obtained and digested, and cells were analysed by flow cytometry. Results: We found a reduced frequency of V delta 1 T cells in tissue from early and late IBD patients (2.24% and 1.95%, respectively, vs 5.44% in healthy tissue) but an increased frequency of V delta 2 T cells in the gut of late IBD patients (3.19% in late patients vs 1.5% in early patients and 1.65% in healthy tissue). The infiltrating V delta 2 T cells had predominant effector memory and terminally differentiated phenotypes and produced elevated levels of tumour necrosis factor-alpha [TNF-alpha] and interleukin-17 [IL-17]. The frequency of tissue V delta 2 T cells correlated with the extent of the inflammatory response and the severity of IBD. Conclusion: Our study shows that tissue V delta 1 T cells are decreased in IBD patients while Vd2 T cells are increased in the gut of IBD patients and contribute to TNF-alpha production. Moreover, we identify an as yet unappreciated role of V delta 2 T cells in IL-17 production in the gut of long-standing IBD patients, suggesting that they also participate in the chronic inflammatory process.

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