Compatibility study of support materials within the enzyme-mediated addressing of proteins

Enzyme-mediated addressing is a versatile, specific, energy-efficient, and easy-to-apply method for the formation of coatings and particle arrangements on surfaces. While variability, with respect to particle materials and achievable structures, has received much attention in the past, the equally important aspect of compatible support materials has not yet been considered. This is however an extremely relevant aspect with regard to real-world applications. In particular, technical polymers and metal supports are of supreme importance in sectors such as life-sciences or nanotechnology. This work is designed as an extensive compatibility study for support materials with the enzyme-mediated addressing of proteins. By careful evaluation of produced coating structures, it is shown that most examined metals and polymers, as well as inorganics and wood, can be successfully coated with thin and highly site-specific protein films, opening up manifold new possibilities for the application of the enzyme-mediated addressing in high-tech products.