期刊
JOURNAL OF CHEMICAL EDUCATION
卷 96, 期 4, 页码 739-744出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jchemed.8b00665
关键词
First-Year Undergraduate/General; High School/Introductory Chemistry; Analytical Chemistry; Curriculum; Laboratory Instruction; Analogies/Transfer; Chromatography; Thin Layer Chromatography; UV-Vis Spectroscopy; HPLC
资金
- Boston University Chemistry Department
- Boston University Grants for Undergraduate Teaching and Scholarship (BU GUTS)
Chromatography is a ubiquitous technique in analytical chemistry, and high-performance liquid chromatography (HPLC) is the industry standard for quantitation of components in solution mixtures. However, demonstrating the principles of liquid chromatography to undergraduates via HPLC-based experiments often proves ineffective because of the fully automated nature of modern HPLC units. This paper details a novel laboratory exercise that is accessible to students in first-year chemistry and high school and helps to demonstrate and demystify the principles involved in HPLC. Using only common and inexpensive laboratory equipment found in most general-chemistry laboratories (burets and benchtop spectrophotometers), students are able to replicate results that are equivalent to those obtained on research-grade HPLC instruments, while gaining valuable insight into chromatography principles.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据