期刊
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷 23, 期 5, 页码 3130-3139出版社
WILEY
DOI: 10.1111/jcmm.14138
关键词
CTCF; FoxO signaling pathway; prostate cancer
资金
- National Natural Science Foundation of China [81700664, 81870525, 81500587, 81572835, 81302234]
- Shandong Provincial Natural Science Foundation, China [ZR2016HP38]
- Projects of Medical and Health Technology Development Program in Shandong Province [2016WS0714]
- Science and Technology Project of Yantai [2016WS018]
The present research focuses on the influence of CCCTC-binding factor (CTCF) on prostate cancer (PC) via the regulation of the FoxO signalling pathway. A bioinfor-matics analysis was conducted to screen out target genes for CTCF in LNCaP cells and to enrich the relevant pathways in LNCaP cells. It was found that the FoxO pathway was enriched according to the ChIP-seq results of CTCF. The expression of CTCF, pFoxO1a, Foxo1a, pFoxO3a and FoxO3a was tested by RT-qPCR and Western blot. Inhibition of CTCF could lead to the up-regulation of the FoxO signalling pathway. The rates of cell proliferation, cell invasion and apoptosis were examined by MTT assay, cell invasion assay and flow cytometry under different interference conditions. Down-regulation of CTCF could suppress cell proliferation, cell invasion and facilitate cell apoptosis. Lastly, the effect of CTCF on tumour growth was determined in nude mice. Inhibition of CTCF regulated the FoxO signalling pathway, which retarded tumour growth in vivo. In conclusion, CTCF regulates the FoxO signalling pathway to affect the progress of PC.
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