期刊
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
卷 95, 期 6, 页码 1409-1412出版社
AMER SOC TROP MED & HYGIENE
DOI: 10.4269/ajtmh.16-0566
关键词
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资金
- IDSA Medical Scholars Program
- University of North Carolina-Chapel Hill Junior Faculty Development Award
- Armed Forces Health Surveillance Center/Global Emerging Infections Surveillance and Response System
- Military Infectious Disease Research Program
The emergence of artemisinin resistance among Plasmodium falciparum in the Greater Mekong subregion threatens malaria control interventions and is associated with multiple unique mutations in K13 (PF3D7_1343700). The aim of this study was to survey Cambodian Plasmodium vivax for mutations in the K13 ortholog (K12, PVX_083080) that might similarly confer artemisinin resistance. Extracted DNA from Cambodian isolates collected between 2009 and 2012 was pooled by province and year and submitted for next-generation sequencing. Single-nucleotide polymor-phisms (SNPs) were identified using a pile-up approach that detected minority SNPs. Among the 14 pools, we found six unique SNPs, including three nonsynonymous SNPs, across six codons in K12. However, none of the SNPs were ortho-logous to artemisinin resistance-conferring mutations in PF3D7_1343700, and nonsynonymous changes did not persist through time within populations. These results suggest a lack of selection in the P. vivax population in Cambodia due to artemisinin drug pressure.
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