期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 24, 期 5, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.24.5.051412
关键词
noninvasive; bioreporter; electromagnetic; thermal; nonthermal
资金
- European Regional Development Fund through the Welsh Government [TG/KJB/VSM: 1063339]
- Ser Cymru National Research Network in Advanced Engineering and Materials [NRN062]
- ENSEIRB (Bordeaux, France)
Biological systems with intrinsic luminescent properties serve as powerful and noninvasive bioreporters for real-time and label-free monitoring of cell physiology. This study employs the bioluminescent marine bacterium Vibrio fischeri to investigate the effects of separated microwave electric (E) and magnetic (H) fields. Using a cylindrical TM010 mode aluminum resonant cavity, designed to spatially separate E and H fields of a pulsed microwave (2.45 GHz) input, we sampled at 100-ms intervals the 490-nm emission of bioluminescence from suspensions of the V. fischeri. E-field exposure (at 4.24 and 13.4 kV/m) results in rapid and sensitive responses to 100-ms pulses. H-field excitation elicits no measurable responses, even at 100-fold higher power input levels (equivalent to 183 A/m). The observed effects on bacterial light output partially correlate with measured E-field-induced temperature increases. In conclusion, the endogenous bioluminescence of V. fischeri provides a sensitive and noninvasive method to assess the biological effects of microwave fields. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.
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