期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 67, 期 10, 页码 2877-2885出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b00228
关键词
Fusarium solani H915; fusarilactones A, B, C; fusaridioic acids B, C; HMG-CoA synthase; tea pathogenic fungus inhibitory effect
资金
- COMRA Project of China [DY135-B2-16]
- National Basic Research Program of China (973 Program) [2015CB755901]
- Xiamen Ocean Economic Innovation and Development Demonstration Project [16PZP001SF16]
- Fujian Key Science and Technology Program [2018N0017]
- Scientific Research Foundation of Third Institute of Oceanography, SOA [2016002, 2017002]
- Xiamen Science and Technology Program [3502Z20172009, 3502Z20182029]
Fusarium solani H915 (MCCC3A00957), a fungus originating from mangrove sediment, showed potent inhibitory activity against tea pathogenic fungus Pestalotiopsis theae. Successive chromatographic separation on an ethyl acetate (EtOAc) extract of F. solani H915 resulted in the isolation of five new alkenoic diacid derivatives: fusarilactones A-C (1-3), and fusaridioic acids B (4) and C (5), in addition to seven known compounds (6-12). The chemical structures of these metabolites were elucidated on the basis of UV, IR, HR-ESI-MS, and NMR spectroscopic data. The antifungal activity of the isolated compounds was evaluated. Compounds with a beta-lactone ring (1, 2, and 7) exhibited potent inhibitory activities, while none of the other compounds show activity. The ED50 values of the compounds 1, 2, and 7 were 38.14 +/- 1.67, 42.26 +/- 1.96, and 18.35 +/- 1.27 mu g/mL, respectively. In addition, inhibitory activity of these compounds against 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase gene expression was also detected using real-time RT-PCR. Results indicated that compounds 1, 2, and 7 may inhibit the growth of P. theae by interfering with the biosynthesis of ergosterol by down-regulating the expression of HMG-CoA synthase.
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