期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 67, 期 15, 页码 4193-4199出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.8b06928
关键词
MmH; amidase; propham; gene cloning
资金
- National Natural Science Foundation of China [31700097]
- Shanghai Sailing Program [17YF1416900, 18YF1420900]
- Domestic Cooperation Program of Shanghai Science and Technology Commission [18295810500]
- Key Agricultural Technology Program of Shanghai Science and Technology Commission [16391901500]
- Shanghai Agriculture Applied Technology Development Program, China [T20180414]
- SAAS Program for Excellent Research Team [2017A-03]
- State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control [2010DS700124-KF1813]
We previously isolated a monocrotophos-degrading strain Starkeya sp. YW6, which could also degrade propham. Here, we show that strain YW6 metabolizes propham via a pathway in which propham is initially hydrolyzed to aniline and then converted to catechol, which is then oxidized via an ortho-cleavage pathway. The novel amidase gene mmH was cloned from strain YW6 and expressed in Escherichia coli BL21(DE3). MmH, which exhibits aryl acylamidase activity, was purified for enzymatic analysis. Bioinformatic analysis confirmed that MmH belongs to the amidase signature (AS) enzyme family and shares 26-50% identity with several AS family members. MmH (molecular mass of 53 kDa) was most active at 40 degrees C and pH 8.0 and showed high activity toward propham, with K-cat and K-m values of 33.4 s(-1) and 16.9 mu M, respectively. These characteristics make MmH suitable for novel amide biosynthesis and environmental remediation.
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