期刊
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
卷 69, 期 3, 页码 708-714出版社
MICROBIOLOGY SOC
DOI: 10.1099/ijsem.0.003207
关键词
Enterobacter; blood; genome
类别
资金
- National Natural Science Foundation of China [81772233, 81661130159]
- Newton Advanced Fellowship, Royal Society, UK [NA150363]
Enterobacter strains 090008(T )and 090028(T) were recovered from the blood of two different patients at West China Hospital, Chengdu, PR China in 2017. Phylogenetic analysis based on the 16S rRNA gene and multi-locus sequence analysis of the rpoB, gyrB, infB and atpD housekeeping genes revealed that the two strains were distinct from any previously known species of the genus Enterobacter. Both average nucleotide identity (ANI) and in silico DNA-DNA hybridization (isDDH) values between strains 090008(T), 090028(T) and type strains of all known Enterobacter species were lower than the cut-off (>= 95-96 % for ANI and >= 70 % for isDDH) to define a bacterial species. The major cellular fatty acids of strains 090008(T)and 090028(T) are C-16 :0, C(17:0 )cyclo and C (18:1)omega 7c, which are similar to other Enterobacter species, and the genomic DNA G+C content was 55.73 and 55.68 mol%, respectively. Strain 090008(T) can be differentiated from other Enterobacter species by its ability to ferment sucrose, melibiose and D-arabitol, but with a negative methyl a-o-mannopyranoside reaction. Strain 090028(T) can ferment potassium gluconate, but is negative for L-fucose, mdecarboxylase, deaminase aelibiose and D-sorbitol, which distinguishes it from all other Enterobacter species. Genotypic and phenotypic characteristics indicate that strains 090008(T) and 090028(T) represent two novel species of the genus Enterobacter, for which the names Enterobacter huaxiensis sp. nov. and Enterobacter chuandaensis sp. nov. are proposed, respectively. The type strain of Enterobacter huaxiensis sp. nov. is 090008(T) (=GDMCC1.1426(T) =CCTCCAB 2018174(T) =CNCTC 7648(T) ) and the type strain of Enterobacter chuandaensis sp. nov. is 090028(T) (=GDMCC1.1427(T) =CCTCCAB 2018173(T) =CNCTC 7649(T)).
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