期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 20, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/ijms20030750
关键词
Triticum aestivum; Landrace; Powdery mildew; Bulked segregant analysis-RNA-Seq (BSR-Seq); Single nucleotide polymorphism (SNP); Kompetitive Allele Specific PCR (KASP)
资金
- National Natural Science Foundation of China [31501310, 31471491]
- National Key Research and Development Program of China [2017YFD0101000]
- Scientific and Technological Research Project of Henan Province of China [172102110110]
- CAAS Innovation Team, Henan Province Young College Key Teacher Subsidy Program [2017GGJS177]
The gene Pm61 that confers powdery mildew resistance has been previously identified on chromosome arm 4AL in Chinese wheat landrace Xuxusanyuehuang (XXSYH). To facilitate the use of Pm61 in breeding practices, the bulked segregant analysis-RNA-Seq (BSR-Seq) analysis, in combination with the information on the Chinese Spring reference genome sequence, was performed in the F-2:3 mapping population of XXSYH x Zhongzuo 9504. Two single nucleotide polymorphism (SNP), two Kompetitive Allele Specific PCR (KASP), and six simple sequence repeat (SSR) markers, together with previously identified polymorphic markers, saturated the genetic linkage map for Pm61, especially in the proximal side of the target gene that was short of gene-linked markers. In the newly established genetic linkage map, Pm61 was located in a 0.71 cM genetic interval and can be detected in a high throughput scale by the KASP markers Xicsk8 and Xicsk13 or by the standard PCR-based markers Xicscx497 and Xicsx538. The newly saturated genetic linkage map will be useful in molecular marker assisted-selection of Pm61 in breeding for disease resistant cultivar and in its map-based cloning.
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