期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 125, 期 -, 页码 711-720出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2018.12.044
关键词
beta-Cyclodextrins; Nano-encapsulation; Periploca angustifolia phenolic extract
资金
- Department of Biology in Faculty of Sciences of Sfax (Tunisia)
The nano-encapsulation of Periploca angustifolia phenolic extract using the macrocyclic carbohydrate polymers (beta-cyclodextrins) is a most approach compared with other encapsulation methods. In this work, the beta-Cyclodextrins-PAE complex stability has been evaluated by advanced analytical methods and techniques including HPLC, FTIR and XRD. The results showed that CdCl2 treatment caused a significant decrease in cell viability. The CdCl2-induced damage in the HepG2 cells were significantly ameliorated (p < 0.001) by treatment of the PAE and beta-Cyclodextrins-PAE complex. Thus, pretreatment with 100 mu g mL(-1) of beta-Cyclodextrins-PAE complex significantly protect HepG2 cells against cytotoxicity induced by cadmium exposure more effectively than PAE only. However, Cd-intoxication significantly (p < 0.001) increased these enzymes activity. Additionally, reactive oxygen species generation was significantly decreased when cells were treated with nano-encapsulation PAE. The levels of supernatant antioxidant parameters including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and GSH were significantly (p < 0.001) decreased in Cd-treated cells with concomitant enhancement of lipid peroxidation. In addition, beta-Cyclodextrins-PAE pretreatment significantly (p < 0.01) inhibited Cd-exposure activated the apoptotic pathway caspace-3 and caspace-9. This effect may be due to the ability of beta-Cyclodextrins molecules to enhance stability and permeability properties. (C) 2018 Published by Elsevier B.V.
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