期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 123, 期 -, 页码 957-967出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2018.11.103
关键词
Sarcandra glabra; Fingerprint analysis; Polysaccharides; Chemometrics analysis
资金
- National Natural Science Foundation of China [81673589, 81473216]
- Qing Lan Project of Jiangsu Province
- 13th of Six Talent Peak Foundation of Jiangsu Province [SWYY-068]
- Open Project of Jiangsu Key Laboratory of Druggability of Biopharmaceuticals [JKLDBKF201703]
- Double First-Class University Project [CPU2018GF08]
- Ministry of Education of China
- State Administration of Foreign Expert Affairs of China [111-2-07]
- Priority Academic Program Development of Jiangsu Higher Educational Institution (PAPD)
Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions. Chemometrics was used to evaluate the similarity and differences of SGPs from different regions based on their fingerprints. The results of the present study showed that polysaccharides from 18 batches of Sarcandra glabra had a high degree of similarity based on the HPSEC, PCD-HPLC, and FT-IR fingerprints. The samples from different regions could be classified by clustering analysis based on their nuances. The five monosaccharides (Gal, Rha, Xyl, GIcA, and Glc) and the wavelengths of FT-IR (3371 cm(-1) and 1411 cm(-1)) could be selected as herb markers for the quality control of Sarcandra glabra. 2018 Elsevier B.V. All rights reserved.
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