期刊
INTERNATIONAL JOURNAL FOR PARASITOLOGY
卷 49, 期 3-4, 页码 311-320出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ijpara.2018.12.003
关键词
Proteome; Parasitic nematode; Haemonchus contortus; Feeding; Metabolism; Development; Parasite-host cross-talk; Immunomodulation
类别
资金
- National Health and Medical Research Council (NHMRC) of Australia
- Australian Research Council
- Melbourne Water Corporation, Australia
- University of Melbourne (BIP), Australia
- Melbourne Bioinformatics Platform, Australia
- NHMRC
Currently, there is a dearth of proteomic data to underpin fundamental investigations of parasites and parasitism at the molecular level. Here, using a high throughput LC-MS/MS-based approach, we undertook the first reported comprehensive, large-scale proteomic investigation of the barber's pole worm (Haemonchus contortus) - one of the most important parasitic nematodes of livestock animals worldwide. In total, 2487 unique H. contortus proteins representing different developmental stages/sexes (i.e. eggs, L3s and L4s, female (Af) and male (Am) adults) were identified and quantified with high confidence. Bioinformatic analyses of this proteome revealed substantial alterations in protein profiles during the life cycle, particularly in the transition from the free-living to the parasitic phase, and key groups of proteins involved specifically in feeding, digestion, metabolism, development, parasite-host interactions (including immunomodulation), structural remodelling of the body wall and adaptive processes during parasitism. This proteomic data set will facilitate future molecular, biochemical and physiological investigations of H. contortus and related nematodes, and the discovery of novel intervention targets against haemonchosis. (C) 2019 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.
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