期刊
HYPERTENSION RESEARCH
卷 42, 期 8, 页码 1166-1174出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41440-019-0243-5
关键词
Endothelial cells; Plasma; Preeclampsia; Resveratrol
资金
- National Council for Scientific and Technological Development (CNPq-Brazil) [2014-5/305587]
- Sao Paulo Research Foundation (FAPESP-Brazil) [2008/53593-0, 2015/20461-8]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Brasil (CAPES) [001]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [08/53593-0, 15/20461-8] Funding Source: FAPESP
In this study, we demonstrated that plasma collected from women who subsequently developed preeclampsia caused increased heme oxygenase-1 (HO-1) production and decreased levels of nitric oxide (NO) markers in endothelial cells (HUVECs). Conversely, no changes in HO-1 or NO markers were found when HUVECs were treated with plasma from women who remained healthy throughout pregnancy. These alterations in HO-1 and NO markers were prevented by cotreatment with the polyphenol resveratrol, which also improved GSH levels. In addition, we evaluated changes induced by plasma incubation in the expression of genes and their related pathways associated with antioxidant defenses, such as Nrf2, ARE activity, and GSR. Collectively, our findings suggest that even before the appearance of clinical symptoms of preeclampsia, plasma from affected women is able to induce modifications in endothelial cells with respect to HO-1 production and NO markers. We believe that this in vitro strategy may offer an attractive alternative to the exploitation of candidate markers or screening molecules, such as resveratrol, for the prevention and management of preeclampsia.
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