期刊
GENETICS
卷 211, 期 4, 页码 1297-1313出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.118.301062
关键词
ADSL deficiency; AICAR; metabolism; purine salvage pathway; SAICAR; SZMP; ZMP
资金
- National Institutes of Health (NIH) Office of Research Infrastructure Programs [P40 OD010440]
- NIH [U41 HG002223]
- AFM-Telethon Trampoline grant [18313]
- AFM-Telethon Ph.D. scholarship [20450]
- Short Term Scientific Mission - GENiE (COST Action) [BM1408]
- Ramon y Cajal program of the Spanish Ministerio de Economia y Competitividad [RYC-2010-06167, RYC-2014-15551]
Purine homeostasis is ensured through a metabolic network widely conserved from prokaryotes to humans. Purines can either be synthesized de novo, reused, or produced by interconversion of extant metabolites using the so-called recycling pathway. Although thoroughly characterized in microorganisms, such as yeast or bacteria, little is known about regulation of the purine biosynthesis network in metazoans. In humans, several diseases are linked to purine metabolism through as yet poorly understood etiologies. Particularly, the deficiency in adenylosuccinate lyase (ADSL)-an enzyme involved both in the purine de novo and recycling pathways-causes severe muscular and neuronal symptoms. In order to address the mechanisms underlying this deficiency, we established Caenorhabditis elegans as a metazoan model organism to study purine metabolism, while focusing on ADSL. We show that the purine biosynthesis network is functionally conserved in C. elegans. Moreover, (the gene encoding ADSL in C. elegans) is required for developmental timing, germline stem cell maintenance and muscle integrity. Importantly, these traits are not affected when solely the de novo pathway is abolished, and we present evidence that germline maintenance is linked specifically to ADSL activity in the recycling pathway. Hence, our results allow developmental and tissue specific phenotypes to be ascribed to separable steps of the purine metabolic network in an animal model.
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